This question was closed without grading. Reason: Other
Dec 23, 2022 17:14
1 yr ago
34 viewers *
English term

balanced with cells

English to German Medical Biology (-tech,-chem,micro-) Arzneimittelwirkung auf Zellen, Krebsforschung
1. Cells were suspended in Dulbecco's modified Eagle medium solution, supplemented with 1% FCSd, then distributed in 384-well plate (100 µL/well) at a density of 5104 cells/well; 2.
Probenecid in Hank's balanced salt solution (HBSS, Invitrogen) supplemented with 20 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (Hepes, Invitrogen) (pH 7.4) was mixed with fluorescent probe (Fluo4 NW, Invitrogen), the mixture was added to each wells, then balanced with cells at 37°C for 60 minutes, and then balanced with cells at 22°C for 15 minutes;


Was versteht mann den hier unter "balanced with"?
Proposed translations (German)
4 cells are suspended in the mixture

Discussion

Lirka Dec 26, 2022:
@Anne: I think you are right! "at 22 C for 15 minutes" is the clue here

Anne Schulz Dec 25, 2022:
Die Erfinder bei diesem Patent sind chinesisch, und ich kann mir eigentlich nur vorstellen, dass sie das falsche Wort erwischt haben und mit "balanced" eigentlich "incubated" meinten. Eventuell wollten sie noch zusätzlich ausdrücken, dass die Menge an Sondenlösung genauso groß ist wie die Menge an Zellsuspension (100 µl/Well)?

Proposed translations

5 hrs

cells are suspended in the mixture

this is my take on it

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Note added at 4 days (2022-12-28 09:59:06 GMT)
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Yes. My translation is more literal, while Anne's reflects the context. If I were you, I'd translate it as I did and add a comment with Anne's explanation for the client.
Note from asker:
Hi, so I think Anne is right and I make a comment to the client. Yes?
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